Depc water dnase. Ultra-pure, proteinase- and pyrogen-free, ready to use.

Depc water dnase. Water is autoclaved both before and after packaging to ensure sterility and to complete inactivation of Other water grades and purity that life sciences, biotech, biopharma, and bioprocess industry refer to are, as follows. Wipe thoroughly with a clean paper towel and then rinse with RNasefree sterile water. Spray with DEPC treated water or RNase Quiet and wait for 1 minute. As mentioned by Ganapati, nuclease free water is a water free of RNase and DNase. It is specifically designed for use in molecular biology applications where the presence of these enzymes could compromise the integrity of DNA or RNA samples. on using DEPC effectively. RNase-Free Water is not treated by DEPC (diethyl pyrocarbonate), and therefore is suitable for applications such as RT-PCR and RNA sample preparation. Nuclease-Free Water is provided in nuclease-free containers. Water, DEPC-Treated is used for the reconstitution of lyophilized products such as oligomers, proteins, and siRNA Aug 16, 2025 · Preparing DEPC Treated Water: A Step-by-Step Protocol The preparation of DEPC treated water requires careful attention to detail to ensure effective RNase inactivation and complete removal of DEPC. Our DEPC-treated water is made using the least amount of DEPC that is still effective at destroying enzymatic activitiy in RNases and other proteins, making it ideal for RNA extraction and all molecular biology applications. Also used for making and running RNA and DNA gels. In practice, milliQ water suffices for most standard PCR (you don't need to buy nuclease free water or DEPC-treat your water). APPLICATIONS • DEPC Treated water is ideal for the preparation of reagents, rinsing glassware and plasticware, and other molecular biology applications where RNase, DNase, and Protease-free water is required. Autoclaving will destroy any unreacted DEPC which can otherwise react with other proteins and RNA. [1][2] DEPC-treated (and therefore RNase-free) water is used in handling of RNA in the laboratory to reduce the risk DEPC treatment is an effective way to eliminate RNAse activity. DEPC treatment should be done in the Hood. ,LTD. Ideal for use in applications such as PCR, qPCR, or RT-PCR, as well as enzymatic reactions where the integrity of nucleic acid is preserved. Prepare the RNA in the following way: Water, (For RNA Work) (DEPC-Treated, DNASE, RNASE free/Mol. Also, don’t use DEPC/DMPC on tris-based solutions. DEPC-Treated water is certified RNase, DNase and Protease free and suitable for use with sensitive RNA research. Jun 8, 2022 · Free from fluorescence particles Free from metal or other ions Visibly clear and odorless Other names: RNase-free water, DNase-free water, molecular grade water and PCR grade water. 1%的DEPC水来做对照呢，为什么？ 因此，在RNA的制备与分析操作实验中，要严格防止RNA酶的污染。 在RNA提取实验前对器材及试剂的处理非常的关键。 焦碳酸二乙酯（DEPC）是一种强烈的RNA酶抑制剂，其通过和RNA酶的活性基团组氨酸的咪唑环结合使RNA酶变性，从而抑制RNA酶的活性。 01 溶液处理 DEPC水：使用DEPC（焦碳酸二乙酯）水去除RNase的水 无DNase水：比较少见，常在无RNase水同时标注 各种纯水根据需要可以高温高压灭菌，DEPC水必需高温高压灭菌，同时DEPC会受热分解。 4 突然发现这问题另一个回答是我之前的号。 。。。 是真的，娃哈哈和屈臣氏确实是 (大学）实验用水。 你跑一下就知道娃哈哈稳过18，这种纯净水也是 反渗透 出来的，大企业管道每天过几万升杂离子早给冲没了， 电导率 都非常好。 反而比有些差点的纯水机干净。 pcr用无酶水。 无酶水经检测无核酸酶和 蛋白酶 活性，可用于cDNA合成、体外转录、RNA提取等对核酸酶敏感的 分子生物学 试验。 DEPC水 是用DEPC (diethypyrocarbonate，焦碳酸二乙酯)处理过并经高温高压消毒的 MiliQ纯水。经检测不含 RNase 、 DNase 和 proteinase。DEPC水可以用于RNA沉淀的溶解，含有RNA的各种反应 . No toxic agents, like DEPC, are used in the water's production. To prevent DNA and RNA sample loss, it is essential that highly pure, nuclease-free water be used in applications such as PCR, cDNA synthesis, nucleic acid purification, sequencing, and cloning. 53 Quantity: Add to Wish List WATER, DNase, RNase-Free, is designed for those critical applications where DNase and RNase contamination can be serious problems. Apr 1, 2025 · DEPC treatment is an effective way to eliminate RNAse activity. 1%的DEPC水来做对照呢，为什么？ 因此，在RNA的制备与分析操作实验中，要严格防止RNA酶的污染。 在RNA提取实验前对器材及试剂的处理非常的关键。 焦碳酸二乙酯（DEPC）是一种强烈的RNA酶抑制剂，其通过和RNA酶的活性基团组氨酸的咪唑环结合使RNA酶变性，从而抑制RNA酶的活性。 01 溶液处理 DEPC水：使用DEPC（焦碳酸二乙酯）水去除RNase的水 无DNase水：比较少见，常在无RNase水同时标注 各种纯水根据需要可以高温高压灭菌，DEPC水必需高温高压灭菌，同时DEPC会受热分解。 4 突然发现这问题另一个回答是我之前的号。 。。。 是真的，娃哈哈和屈臣氏确实是 (大学）实验用水。 你跑一下就知道娃哈哈稳过18，这种纯净水也是 反渗透 出来的，大企业管道每天过几万升杂离子早给冲没了， 电导率 都非常好。 反而比有些差点的纯水机干净。 pcr用无酶水。 无酶水经检测无核酸酶和 蛋白酶 活性，可用于cDNA合成、体外转录、RNA提取等对核酸酶敏感的 分子生物学 试验。 DEPC水 是用DEPC (diethypyrocarbonate，焦碳酸二乙酯)处理过并经高温高压消毒的 MiliQ纯水。经检测不含 RNase 、 DNase 和 proteinase。DEPC水可以用于RNA沉淀的溶解，含有RNA的各种反应 DEPC水：使用DEPC（焦碳酸二乙酯）水去除RNase的水 无DNase水：比较少见，常在无RNase水同时标注 各种纯水根据需要可以高温高压灭菌，DEPC水必需高温高压灭菌，同时DEPC会受热分解。 4 突然发现这问题另一个回答是我之前的号。 。。。 DEPC处理没有消除核酸酶，因此很难说DEPC水是“无酶”的，且DEPC残留及分解产物（如乙醇）对于部分实验可能有影响，所以DEPC水和各种无酶水在部分公司的产品中是被区分的，如 ThermoFisher [1] [2]。 无核酸酶水可以通过超滤制备 [3]。 参考 如果使用一般的水或缓冲液，可以在使用前通过高温灭菌或使用DEPC（乙二胺四乙酸）处理进行去RNase。 2. com. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Nuclease-Free Water DNase-Free, RNase-Free, Protease- Free Not DEPC Treated 0. Methods of removing active nucleases include filtration and treatment with Water, DEPC-Treated, is a DEPC(Diethylpyrocarbonate)-treated and sterile filtered water. Sep 2, 2025 · Nuclease Free water. We are doing the procedure since last 5 years and never used DNAse, instead use proper DEPC treated plasticware and dissove the RNA in DEPC water. Cover it with parafilm and shake well until DEPC dissolves in water. DEPC-treated water is certified nuclease-free and supplied in four bottles containing 1 L each to ensure sterility and inactivation of DEPC. UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. This product (s) resides on a Fisher Scientific GSA or VA contract. DEPC-treated Water - DEPC destroys enzymatic activity by modifying -NH 2, -SH and -OH groups in RNases and other proteins. DEPC-Treated Water Features Clear, Liquid Solution DNase & RNase-Free Commonly used in molecular biology and biochemistry applications Free of impurities Aug 14, 2025 · PurMa™ Biologics DEPC Treated Ultra-Pure Nuclease Free Water is suitable for very sophisticated molecular biology applications and it is DNase/RNase Free. Features: 1. 当前位置： 首页> 产品中心> 分子生物学研究 > RNA提取 > DEPC-treated Water (DNase、RNase free) DEPC水（无DNase、RNase ) DEPC-treated Water is highly pure and nuclease-free (tested for both contaminating nonspecific DNase and RNase activities), specially developed for all RNA work. Water, DEPC-Treated is used for the reconstitution of lyophilized products such as oligomers, proteins, and siRNA. It can be on a UV-sterilized flow hood I know that DEPC treatment can make water RNase free. DEPC-treated, DNase- & RNase-free options available. 1% (v/v) DEPC in water or ethanol for 1 hour, followed by draining and autoclaving. 1% final concentration) to 1 l of sterile water at 37°C overnight under fume hood and then autoclave Footnote 3 10 U/μl DNaseI FPLC pure (cod. 4. 1% DEPC (diethyl pyrocarbonate-Sigma, cat {{title. For 2 liters of buffer, add 83. DEPC treatment is a very effective way to treat solutions that will contact RNA. DEPC Treated Water, Ultra Pure, DNase, RNase and Protease Free, 10X1L/Case, 351-068-131CS DEPC Treated Water is Ultra Pure and prepared by reverse osmosis, passed through fine carbon, de-ionized through two resin beds and serially filtered 1µm positively charged membranes. Molecular Grade Water™ is suitable for use in molecular biology applications which demand a high quality of water and assurance that the water is free from DNase, RNase, and protease contamination. Note heating variations in the chart below. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water. no. Contains very low chloride, cation, and endotoxin levels This water is prepared by May 27, 2025 · Then, you autoclave the treated water and the DEPC is gone, leaving carbon dioxide, ethanol and a little bit more water behind (DEPC decays spontaneously and, as any energy-dependent reaction, heating it up speeds up the process). Shop for DEPC-treated water. DEPC-Treated Water only inactivates RNases that are present in the water when it is made, but won’t inactivate RNases that are introduced after it has been autoclaved. This water is ideal for sensitive molecular biology applications requiring nuclease-free conditions, including RNA experiments, cDNA synthesis, and RT-PCR. Performance and quality testing Jul 12, 2025 · DEPC treatment is an effective way to eliminate RNAse activity. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. Description Water, RNase, DNase-free high quality, RNase/DNase-free water is used in all molecular biology applications like PCR, DNA. Some documentation UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. Learn from NINGBO INNO PHARMCHEM CO. Features and Benefits Nuclease-Free Water DNase-Free, RNase-Free, Protease- Free Not DEPC Treated 0. 1% DEPC（diethypyrocarbonate，焦碳酸二乙酯）处理，再经高温高压消毒的MiliQ纯水。经检测不含RNase、DNase和proteinase。 DEPC水可以用于RNA沉淀的溶解，含有RNA的各种反应体系如反转录、siRNA的退火等。 DNase/RNase-Free Deionized Water is designed for use in all molecular biology applications and provided in a convenient 1-liter and 2-liter bottles. Learn the different methods with this article. ), Fisher BioReagents from Fisher BioReagents. Available in 1 L 本产品不含核酸酶、蛋白酶及DEPC。 本产品DNase<5pg/ml、RNase<1pg/ml，实际测试37℃孵育2小时检测不到明显的RNA或DNA降解。 经检测本产品中的RNase去除效果与DEPC处理的效果相当，同时避免了自行使用DEPC处理时可能带来的DEPC残留而导致的后续对于一些酶反应的抑制效应。 Learn how DEPC-treated water is crucial for RNase-free RNA extraction. For more information, please contact our customer service department. DEPC-Treated Water is Ultra Pure and prepared by reverse osmosis, passed through fine carbon, de-ionized through two resin beds and serially filtered twice through 0. Next day Autoclave for 30 minutes at 121 degree celsius. There remains a fair amount of resistance to using DEPC in the laboratory. May 9, 2020 · Nuclease-free water is free of both DNAse and RNAse, and involves treating with DEPC (diethylpyrocarbonate) and/or autoclaving to inactivate RNAse and DNAse. 129117). 1. 2 micron filter. No DEPC treated 上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念 Explore nuclease-free water for PCR, dPCR, and cfDNA synthesis. Liquid is RNase and DNase Free. Molecular grade water, DNase, and RNase free water, and DEPC-treated water; these nomenclatures signify that water is suitable for molecular biology applications to protect DNA and RNA from degrading contaminating nucleases. Nuclease-Free Water is prepared in a proprietary process, which yields DNase, RNase, and nuclease-free, deionized water without the use of chemical additives, such as diethylpyrocarbonate (DEPC). Ultra-pure deionized water passes through hollow fiber ultrafiltration column to reduce the level of heat source, endotoxin and nuclease. 1-µm membrane in a clean environment. Available in 1 L Fisher BioReagents Water, Molecular Biology Grade is ideal for many fundamental procedures such as PCR, electrophoresis, DNA sequencing and buffers for enzymatic analyses. It also does not contain any nucleases. Decontaminate glassware by baking at 180°C or higher for several hours or by soaking in freshly prepared 0. Shop Invitrogen™ DEPC-Treated Water at Fishersci. 操作人员要注意 佩戴手套：RNA酶广泛存在于人体皮肤上，因此操作RNA时，操作人员应佩戴一次性手套，避免直接接触RNA样本。 Apr 27, 2023 · 在实验中，DEPC通常用于处理水、试剂、玻璃器皿等，以消除其中的核酸酶污染；DEPC还可以与蛋白质中的氨基酸发生反应，形成羧甲基衍生物，从而保护蛋白质不受降解。 因此，在蛋白质样品的储存和处理过程中，可以添加0. 碧云天生产的DEPC水，即DEPC-treated Water，是用DEPC (diethypyrocarbonate，焦碳酸二乙酯)处理过并经高温高压消毒的Milli-Q纯水。经检测不含RNase、DNase和proteinase。 Aug 26, 2025 · DEPC treatment is an effective way to eliminate RNAse activity. Water, DEPC-Treated, is a DEPC (Diethylpyrocarbonate)-treated and sterile filtered water. Discover preparation and best practices from a leading chemical supplier. This water is typically pre-treated to remove ions and organic contaminants. Invitrogen DEPC-Treated Water from Invitrogen™. Get more details at Azer Scientific. 1 um Filtered Prepared from Double Distilled and Deionized Water Suitable for use in Molecular Applications This product is 0. Ambion DEPC-treated water is certified nuclease-free. If I use DEPC-treated water for making PBS, I think, at the time of checking the pH the solution may be contaminated with RNase. On the RNA extraction day, have a checklist with all the materials and solutions ready in a clean and aseptic place. Nuclease-Free Water is no longer available in a 5 liter bag with luer cap (cat. Our distilled water system is routinely monitored for compliance with current USP monograph test requirements for Water for Injection (WFI). Water Sterile-filtered water treated with diethyl pyrocarbonate (DEPC), molecular biology grade CAS Number: 7732-18-5; Synonyms: Water; Linear Formula: H2O at Sigma-Aldrich DEPC-Treated Water（DNase、RNase free），Sterile，500mL/ Bottle, 10 Bottles/Case Specifications General Details CAS # SMILES Chemical Purity Molecular Formula Chemical Formula Formula Weight Reaction Note Physical Properties Purity Density Melting Point Boiling Point Flash Point Vapor Pressure Surface Area Sep 10, 2022 · Nuclease-free water is free of both DNAse and RNAse, and involves treating with DEPC (diethylpyrocarbonate) and/or autoclaving to inactivate RNAse and DNAse. It is ready to use and requires no preparation, mixing, or autoclaving. No toxic agents, such as DEPC, are used in the manufacturing of Molecular Grade Water™, eliminating DEPC interference of enzymatic reactions. 001% diethylpyrocarbonate (DEPC). Each lot is quality tested for the absence of RNase, DNase and protease contamination. Available in 1 x 100 mL Many experimental procedures in biological research require the use of RNase-free water to prevent the degradation of RNA. Ensure all glassware and containers are clean and RNase For RNAse free water I use Ambicon DEPC-treated water (autoclaved and filtered, packaged and autoclaved again), it has no "DEPC smell"-but see #3 below. Apr 25, 2019 · Soak in a 0. Water, Molecular Biology Grade, Sterile RNase Free, DNase Free, Protease Free, DEPC Free, 1L Part Number: R9145000-1G Volume: 1L Product Type: Water Product Style: Molecular Biology $47. DEPC treated to inactivate RNases. This nuclease-free water is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity. Use barrier tips. 1um filtered. Ideal for working with RNA. Filtered and autoclaved to provide a Invitrogen DEPC-Treated Water from Invitrogen™. Water is autoclaved both before and after packaging to ensure sterility and to complete inactivation of Method Pretreat all the plastic, glassware, mortar and pestle with 0. Prepare all solutions and buffers with RNase-free glassware, DEPC-treated water, and chemicals reserved for work with RNA that are handled with disposable spatulas or dispensed by tapping the bottle rather than using a spatula. Apply 50 µl RNA Solution (40 µg/ml) on the cover glasses and incubate them at 37°C for 30 minutes. Water is autoclaved both before and after packaging to ensure sterility and to complete inactivation of The presence of nucleases such as DNase and RNase in water can degrade precious molecular samples and even ruin experiments. Alternatively, an ultrafiltration cartridge can be placed at the outlet of a water purification system in order to deliver nuclease-free ultrapure WATER, DNase, RNase-Free, is designed for those critical applications where DNase and RNase contamination can be serious problems. Overview DEPC-treated water is a molecular biology grade water obtained by diethylpyrocarbonate (DEPC) treatment and autoclaving of ultra-filtrated water. If it wasn't, the water would be useless since it's a pretty non-specific inhibitor of enzymes. Ultra-pure DEPC-Treated water is suitable for use with sensitive RNA research. 碧云天生产的DEPC水，即DEPC-treated Water，是用DEPC (diethypyrocarbonate，焦碳酸二乙酯)处理过并经高温高压消毒的Milli-Q纯水。经检测不含RNase、DNase和proteinase。 Diethylpyrocarbonate (DEPC)-treated Water is guaranteed nuclease-free (tested for both DNase and RNase) and is suitable for all RNA work. Key features of WFI include its DNase and RNase-free quality, ensuring that no enzymatic degradation occurs in nucleic acid studies. Nuclease-free glassware and reagents are required for many molecular biology applications. Carefully clean the surfaces. DEPC efficiently inhibits RNases by covalent modification. For use in molecular biology applications, PCR reactions, 2D electrophoresis, and mass spectrometry. No toxic agents, such as DEPC, are used in the manufacturing of Molecular Grade Water, eliminating DEPC interference of enzymatic reactions. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity, protease. Suitable for use with RNA. DEPC treated water is ultrapure water treated with DEPC (diethyl pyrocarbonate) and sterilized by high temperature and high pressure. DEPC treatment is an effective way to eliminate RNAse activity. No toxic agents, such as DEPC, are used in Nuclease-free water for molecular biology applications including PCR, dPCR and cfDNA synthesis. Water, RNase-free, DEPC Treated, Molecular Biology Grade, Ultrapure. 27-0514 GE Healthcare) Explore nuclease-free water for PCR, dPCR, and cfDNA synthesis. DEPC-treated water is autoclaved pre- and post-packaging to ensure sterility and inactivation of DEPC. 23g sodium acetate to 1. For research use only. Meets WFI quality Standards although not intended for use as an injectable. There are many ways to remove contaminants, such as RNase and DNase, in your plastic containers. Filtered through 0. DNase/RNase and pyrogen free. 1% aqueous solution of diethyl pyrocarbonate 2 (DEPC) for 2 hours at 37°C (99°F); rinse several times with sterile (DEPC-treated) water*; heat to 100°C (212°F) for 15 minutes or autoclave for 15 minutes at 121°C (250°F) on a liquid/slow exhaust cycle. DEPC water (DNase/RNase free) is a type of ultrapure water that has been treated with diethylpyrocarbonate (DEPC) to inactivate DNase and RNase enzymes. Available in 10 x 50 mL Water, RNase, DNase-free high quality, RNase/DNase-free water is used in all molecular biology applications like PCR, DNA. DEPC is evaporated off by autoclaving after treatment. May 11, 2022 · For example, the use of RNase inhibitors, RNA stabilizers and DEPC water etc. Nuclease-free: DEPC-treated water undergoes a meticulous purification process involving reverse osmosis, deionization, filtration, DEPC treatment, and autoclaving, followed by stringent testing for various contaminants, including DNases, and RNases. Diethylpyrocarbonate (DEPC)-treated Water is guaranteed nuclease-free (tested for both DNase and RNase) and is suitable for all RNA work. DEPC inactivates RNase enzymes in water by covalent modifications of the histidine residues. To obtain nuclease-free water you will need to treat your water with 0. 1% (v/v) DEPC (Diethylpyrocarbonate) and incubated and heated overnight at 37° Many experimental procedures in biological research require the use of RNase-free water to prevent the degradation of RNA. Available in 10 x 1 mL The high quality DNAse & RNAse Free water is indicated for reagent preparation in nucleic acid research. Ultrapure water, on the other hand, is obtained by ultrafiltration to achieve the high level of purity. DNase/RNase-Free Distilled Water (Non-DEPC Treated) is ideal for procedures sensitive to the presence of nuclease enzymes. Identification Water, RNase-free, DEPC treated, Molecular Biology Grade, Ultrapure, Thermo Scientific Apr 10, 2025 · It is certainly true but higher DEPC concentrations mean that there is likely to be more left-over DEPC/DEPC by-products after autoclaving, and these can inhibit downstream applications. Synonyms: Diethyl pyrocarbonate (DEPC)-treated water is guaranteed nuclease-free (tested for both DNase and RNase) and is suitable for all RNA work. The water is subsequently treated with 0. This product removes RNase, DNase, and various other organic and inorganic substances by reverse osmosis, ion exchange, and ultrafiltration. DEPC is the substance used to purify the (already bidistilled) water itself, because DEPC inhibits nucleases; in the final step of preparing DEPC-treated water you autoclave it, and DEPC degrades ChemicalBook 致力于为化学行业用户提供DEPC水 (DNASE、RNASE FREE)的性质、化学式、分子式、比重、密度,同时也包括DEPC水 (DNASE、RNASE FREE)的沸点、熔点、MSDS、用途、作用、毒性、价格、生产厂家、用途、上游原料、下游产品等信息。 Jun 4, 2025 · DEPC treatment is an effective way to eliminate RNAse activity. 100, 500, and 1,000 ng/ml RNase A was added to water followed by various amounts of DEPC. DEPC is effective as a nuclease inhibitor. Add 20ml of DEPC-treated 0. Available in 25 mL UltraPure™ DEPC-treated Water is suitable for use with RNA. Nuclease-free Water is essential to preserve the sample integrity for applications such as PCR, cloning, sequencing, or gene editing. Discover its features, packaging, and lab storage instructions. This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Aug 28, 2025 · DEPC treatment is an effective way to eliminate RNAse activity. 1% DEPC (diethylpyrocarbonate), an RNase inhibitor, and then autoclaved to remove the DEPC, to prevent downstream interference, and sterilized. DEPC-Treated water is certified RNase, DNase, and Protease free. Preparation: Use high-quality, molecular biology grade water. CAS: 7732-18-5 Research Use Only. Water is autoclaved both before and after packaging to ensure sterility and to complete inactivation of DEPC水：使用DEPC（焦碳酸二乙酯）水去除RNase的水 无DNase水：比较少见，常在无RNase水同时标注 各种纯水根据需要可以高温高压灭菌，DEPC水必需高温高压灭菌，同时DEPC会受热分解。 4 突然发现这问题另一个回答是我之前的号。 。。。 DEPC处理没有消除核酸酶，因此很难说DEPC水是“无酶”的，且DEPC残留及分解产物（如乙醇）对于部分实验可能有影响，所以DEPC水和各种无酶水在部分公司的产品中是被区分的，如 ThermoFisher [1] [2]。 无核酸酶水可以通过超滤制备 [3]。 参考 如果使用一般的水或缓冲液，可以在使用前通过高温灭菌或使用DEPC（乙二胺四乙酸）处理进行去RNase。 2. Functions of nuclease-free water: Ideally, all the molecular procedures in which nucleic acid- DNA or RNA is involved, use only certified nuclease-free water. Water is autoclaved both before and after packaging to ensure sterility and to complete inactivation of Ultra-pure DEPC-Treated water is suitable for use with sensitive RNA research. View or download the DNase I, RNase free MSDS (Material Safety Data Sheet) or SDS for 69182 from Merck. The report documents that the efficiency of an in vitro transcription reaction decreases with as the DEPC concentrations used to treat the water are increased. RNase-Free Water is prepared with a proprietary process, which yields RNase-free, deionized water without the use of chemical additives, such as diethylpyrocarbonate (DEPC). DNase/RNase-free water | Nuclease-free water 2. DEPC chemical treatment is often used to eliminate the nucleases from solutions as it is an efficient inhibitor of DNase and RNase. Water, for molecular biology, DNAse, RNAse and Protease free. Ambion DEPC-treated water is certified nuclease-free and supplied in five bottles containing 100 mL each. Thermo Scientific Water RNase-free DEPC Treated Molecular Biology Grade. Similarly, prepare all the solutions with autoclaved DEPC-treated water. Sterile filtered. Learn more about DEPC H20 on our site. Explore nuclease-free water for PCR, dPCR, and cfDNA synthesis. 5M EDTA and adjust the pH to 7. Water, DEPC-Treated is used for the reconstitution of lyophilized products such as oligomers, proteins, and siRNA DEPC Water is sterile water treated to eliminate RNase and DNase. Sep 3, 2025 · Our DEPC-treated water is made using the least amount of DEPC that is still effective at destroying enzymatic activitiy in RNases and other proteins, making it ideal for RNA extraction and all molecular biology applications. DEPC水，即DEPC-treated Water，是用DEPC (diethypyrocarbonate，焦碳酸二乙酯)处理过并经高温高压消毒的Milli-Q纯水。经检测不含RNase、DNase和proteinase。 DEPC水可以用于RNA沉淀的溶解，含有RNA的各种反应体系如反转录、siRNA的退火等，以及其它各种要求无RNase、DNase和proteinase的反应体系。 DEPC水不同于DEPC。DEPC水是 DEPC-treated Water - DEPC destroys enzymatic activity by modifying -NH 2, -SH and -OH groups in RNases and other proteins. UltraPure molecular biology grade water is treated with DEPC followed by autoclaving to specifically degrade RNases and is highly suitable for RNA applications. Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity. 1%的DEPC来保护蛋白质的完整性和稳定性。 Jul 16, 2021 · 双蒸水也可以这样处理，但DEPC残留和分解产物对于一些实验有影响，建议先充分了解实验用水需求。 以及，RNase Free ddH2O这样的产品是可以直接买的。 我的细胞实验是一个实验组一个对照组，实验组用抗体处理，对照组可不可以用相同体积的0. Performance and quality testing No DNase, RNase, or protease activity detected. DEPC-treated water is ideal for molecular biology procedures where DNase and RNase activity must be absent. That is enough to get rid of RNA contaminations. DEPC-Treated water for PCR and other applications. RNase-free water is generated when distilled water is treated with diethylpryrocarbonate (DEPC), which efficiently inhibits RNases by covalent modification. It is prepared by incubating with 0. It does so by the covalent modification of histidine (most strongly), lysine, cysteine, and tyrosine residues. Performance and Quality Testing: No DNase or RNase activity detected. Water is usually treated with 0. Designate a workspace, and a set of pipettes, if possible, that are dedicated to RNase-free work. Chemical treatment with diethylpyrocarbonate (DEPC) has long been a common practice to generate nuclease-free water, but this method has several drawbacks. UltraPure™ DEPC-treated Water is suitable for use with RNA. DNase/RNase-Free Deionized Water is designed for use in all molecular biology applications and provided in a convenient 1-liter and 2-liter bottles. RNase-Free Water is provided in RNase-free containers. Functionally there is no difference between DEPC treated water and water made nuclease free by any other means. Using Nuclease-free Water Because this water is not DEPC-treated, it is recommended for DEPC treated water is ultrapure water treated with DEPC (diethyl pyrocarbonate) and sterilized by high temperature and high pressure. Reduce the cost of every drop of molecular grade water your lab uses with this low cost of ownership system. The product is certified for the absence of DNases, RNases, and proteases and is made sterile by filtering through 0. It has been tested to be free of RNase, DNase and proteinase. 1% (v/v) DEPC (Diethylpyrocarbonate) and incubated and heated overnight at 37°C. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. 02 SKU: MND-3496 CAS Number: 7732-18-5 MDL number: MFCD00011332 EC Number: 231-791-2 Form: liquid Pricing Packing size: 1 L Request a quote 赛默飞无核酸酶水和DEPC处理水经过严格测试和认证，Invitrogen水不含核酸酶，适用于核酸分离和分析。Thermo Fisher无核酸酶水和 DEPC处理水，为您的RNA研究提供可靠支持。 DEPC-treated (and therefore RNase-free) water is used in handling of RNA in the laboratory to reduce the risk of RNA being degraded by RNases. It can be used instead of DEPC treated water. DEPC-Treated Water (DNase/RNase Free) Introduction DEPC treated water is commonly used for preparing 75% ethanol, RNase Free reagents and dissolving RNA. Nuclease-free Water is ideal for enzymatic reactions and for preparing reagents. Often known as ‘DEPC-treated water’ is prepared in the lab and used to wash common glass and plasticware for the RNA isolation. Water is autoclaved both before and after packaging to ensure sterility and to complete inactivation of DEPC-treated and autoclaved Diethylpyrocarbonate (DEPC)-treated Water is guaranteed nuclease-free (tested for both DNase and RNase) and is suitable for all RNA work. Jul 7, 2022 · Nuclease- free water is free of both DNAse and RNAse, and involves treating with DEPC (diethylpyrocarbonate) and/or autoclaving to inactivate RNAse and DNAse. DEPC is so important in this process. Water, DEPC-Treated, Molecular Biology Grade - CAS 7732-18-5 - Calbiochem Sterile-filtered water treated with diethyl pyrocarbonate (DEPC). Not for use in diagnostic procedures. DEPC is a potent, non-specific chemical inhibitor of RNase 2 and its most common application has been to make water nuclease-free. 0 with 10N NaOH. DEPC H20 is prepared by incubating with 0. Add 2. This product is also confirmed to be DNase-free. Autoclaved to yield a sterile solution. May 16, 2020 · 2) Often people do DEPC treatments just to be completely sure - since proving that water is completely nuclease free is ether difficult or takes more labour that the DEPC treatment. Then cover with Aluminium foil loosely and shake to wet the rim of the flask and leave it in the hood for Overnight. People are very fond of treating water with DEPC (diethylpyrocarbonate) to make it RNase free but that is a waste of time because the water is among the least likely sources of RNases in labs. 1% diethylpyrocarbonate (DEPC), and is then autoclaved to remove the DEPC. Water, DEPC-Treated is used for the reconstitution of lyophilized products such as oligomers, proteins, and siRNA A comprehensive guide to preparing DEPC-treated water, its role in RNA isolation, and essential safety precautions. Ultra-pure, proteinase- and pyrogen-free, ready to use. Ambion's DEPC-treated water is autoclaved both before and after packaging to ensure sterility and complete inactivation of DEPC. Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. 0 ml of DEPC to 2500ml of 17. This product has been analyzed for the absence of nucleases and proteases, and RNase/DNase-free Water, non DEPC can be used in various molecular biology experiments including RNA experiments. DEPC-treated sterile water, DNase- and RNase-free, 500 mL. Biol. Recently we use DEPC water only for RNA work. WATER, DNase, RNase-Free, is designed for those critical applications where DNase and RNase contamination can be serious problems. 1-μm membrane-filtered and tested for DNase and RNase activity. 1% v/v DEPC for at least 2 hours at 37 °C and then autoclaved (at least 15 min) to inactivate traces of DEPC. Not DEPC-treated. Shop Growcells Water - DEPC Treated, Nuclease Free at Thomas Scientific, your trusted partner in Science. DEPC-treated and autoclaved Diethylpyrocarbonate (DEPC)-treated Water is guaranteed nuclease-free (tested for both DNase and RNase) and is suitable for all RNA work. 1μm filtration, followed by sterilization. Nuclease-free water to prevent or minimize the degration of nucleic acids in life science workflow. It is 0. - Find MSDS or SDS, a COA, data sheets and more information. Synonym: DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water Molecular Formula: H2O Molecular Weight: 18. 6L of DEPC-treated water and stir until completely dissolved. It reacts with many enzymes containing -NH, -SH DEPC-treated Water - DEPC destroys enzymatic activity by modifying -NH 2, -SH and -OH groups in RNases and other proteins. 1% DEPC-treated, Milli-Q water and autoclave. meta|trust}}" DNase treatment The DNase cocktail consists of the following (per sample): RQ1 RNase free DNase: 1 μl DNase 10X reaction buffer: 2 μl DEPC-treated H2O: 6 μl RNase Out: 0. ), Fisher BioReagents™ at Fishersci. Molecular Grade Water is suitable for use in molecular biology applications which demand a high quality of water and assurance that the water is free from DNase, RNase and protease contamination. Rnase, nuclease free water for labs. DEPC is also carcinogenic and should be handled with care and with strict attention to the safety recommendations of the manufacturer. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen. 5 megaohms water. If you use use DEPC it is hygroscopic - that is abosrbs water dilutin it - so should be stored in the dark with parafilm around the lid and replaced every 2-3 months Explore nuclease-free water for PCR, dPCR, and cfDNA synthesis. Apr 10, 2025 · Note that ultrafiltered water is already RNase free so does not need DEPC treatment. Click here to order. DEPC-treated and autoclaved Shop Water, (For RNA Work) (DEPC-Treated, DNASE, RNASE free/Mol. Oct 16, 2017 · DEPC水（无DNA酶、RNA酶） DEPC-treated Water (DNase-free, RNase-free) 产品货号：JY05011 规格：100 mL 销售价格：¥50 产品货号：JY05012 规格：500 mL 销售价格：¥100 DEPC水（无DNA酶、RNA酶） 说明书. Alternatively, an ultrafiltration cartridge can be placed at the outlet of a water purification system in order to deliver nuclease-free ultrapure DEPC水（DEPC-treated Water ）是用0. If you are viewing this page as a nonregistered user, the price (s) displayed is List Price. It is widely used for several fundamental procedures like PCR, gel electrophoresis, DNA sequencing etc. It decomposes at 155°C. Apply 1 µl ethidium bromide solution (20 µg/ml) on the cover glasses with a pipette. Deionized water treated with 0. Water is autoclaved both before and after packaging to ensure sterility and to complete inactivation of UltraPure DEPC-treated Water is suitable for use with RNA. 3. 5 μl Make a master mix of the above based on the number of RNA samples being treated. (Note: DEPC cannot be used with certain buffers. Aug 21, 2023 · Cost-effective: PCR water packed in 2ml vials, designed specifically for PCR work, avoid wasting large capacity reagents and save costs Exquisite Craftsmanship: The purification process included continuous deionization, reverse osmosis, UV-treatment, 0. Inactivation of DEPC in this manner yields CO2 and ethanol. But there is a process to use it, can’t be used directly. FALSE, but the amount of DEPC required to inactivate RNase increases as the amount of contaminating RNase in a solution increases (Figure 3). Previously we used to use RNAse free water (DEPC water) for all genetic experiments that is RNA extraction, cDNA synthesis, semi-PCR or RT-PCR. 2 μm positively charged membranes. (Heating or autoclaving will remove DEPC residues). Water is autoclaved both before and after packaging to ensure sterility and to complete inactivation of WATER, DNase, RNase-Free, is designed for those critical applications where DNase and RNase contamination can be serious problems. pdf 产品简介 本产品是经DEPC（diethylpyrocarbonate，焦碳酸二乙酯）处理过并高温高压灭菌的超纯水，经检测不含RNase、DNase和 Jan 1, 2011 · All reagents should be RNase free 2 and DEPC treated DEPC-treated water (DEPC H 2 O): add 1 ml of diethylpirocarbonate (DEPC) (0. DEPC Water is sterile water treated to eliminate RNase and DNase. Clean equipment with a Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. RNase Quiet is a ready-to-use solution for eliminating DNase and RNase as well as DNA and RNA by wiping glass, plastic lab ware, bench surfaces, and other surfaces. Diethylpyrocarbonate (DEPC)-treated Water is guaranteed nuclease-free (tested for both DNase and RNase) and is suitable for all RNA work. Diethyl pyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase enzymes in water and on laboratory utensils. DEPC treatment is the most commonly used method for eliminating RNase contamination from water, buffers, and other solutions. May 3, 2025 · DEPC treatment is an effective way to eliminate RNAse activity. 72g of MOPS (free acid) and 8. Find out the procedure for RNA isolation, DNase treatment and reverse transcription in our detailed protocol for cells in culture and tissue samples. DEPC water, a form of WFI, is treated with diethyl pyrocarbonate, making it ideal for molecular biology experiments, particularly those involving sensitive processes such as RNA and DNA research. Water is autoclaved both before and after packaging to ensure sterility and to complete inactivation of Sterile water DNase RNase Protease Free DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water Tested in accordance to testing for “Sterile Purified Water”, United States Pharmacopeia. Options are diethyl pyrocarbonate (DEPC) treated water, DNase- and RNase-free water. Jan 15, 2015 · UltraPure molecular biology grade water is treated with DEPC followed by autoclaving to specifically degrade RNases and is highly suitable for RNA applications. DEPC-Treated Water is ultrapure, nuclease-free water treated with diethyl pyrocarbonate (DEPC) to inactivate RNase and DNase enzymes. Product Description: DEPC Molecular formula: C6H10O5 Diethyl Pyrocarbonate (DEPC) is sensitive to moisture and to pH; it decomposes to ethanol and carbon dioxide in aqueous solution. Performance and Quality Testing: No DNase Water, DEPC-Treated, is a DEPC (Diethylpyrocarbonate)-treated and sterile filtered water. rc7g8 lq oo7 oqe 0fwv3 5qrnl 6jz8ces up8 l9d zi